### Nitrite Test Kit

**Source of Nitrogen Compounds:**
Food leftovers, fish excretion products, plant debris, and dead fish are broken down into ammonia by heterotrophic bacteria in a process called ammonification. Much of the ammonia also originates directly from fish, mainly excreted through their gills. In the sequence of this process, ammonia, through the action of two groups of nitrifying autotrophic bacteria, is first oxidized to nitrite and then to nitrate.

This final compound is ultimately consumed as a nutrient by algae and aquatic plants. In intact natural environments, there is a complete balance between the organisms and the compounds generated, ensuring this cycle works perfectly. Since tanks are limited environments, the desired balance is not usually achieved naturally and spontaneously.

Monitoring nitrogen compounds (ammonia, nitrite, and nitrate) in the water, as well as maintaining these compounds, should be performed by specialized personnel, as their proper use ensures better tank performance.

High levels of nitrite are toxic to fish, causing stress and affecting the red blood cells, reducing their respiratory capacity. Tank water under such conditions, for a prolonged period, can cause fish to die from asphyxiation. Adequate nitrite levels do not necessarily mean adequate levels of its precursor ammonia and vice versa, hence the need to monitor both variables.

**Instructions for Use:**
1. Using the test tube, collect water from the tank to be analyzed, up to the mark.
2. Add 2 drops of reagent solution 1, cap the tube, and shake. For saltwater, use 1 drop.
3. Add 2 drops of reagent solution 2, cap the tube, and shake. For saltwater, use 1 drop.
4. After 10 minutes, compare the developed color with the color scale, preferably under natural light. For better visualization, place the open test tube on the scale and read from above.

**Important:**
When high levels of nitrite are detected, a partial water change should be performed immediately.

**Notes:**
- After each test, immediately wash the test tube and keep it closed. If the test tube is not clean, the color in the next test may be altered, resulting in reading errors.
- To ensure better efficiency of the tests, keep the reagent bottles tightly closed.

**Measures to Prevent High Nitrite Levels in Aquariums:**
- Control the amount of food to avoid leftovers.
- Avoid overpopulation of fish.
- Install and maintain a proper biological and mechanical filtration system.
- Perform partial water changes and filter cleaning as needed.

**Number of Tests:**
- 100 tests.

If you have any questions about the product, we will be happy to assist you.
- Email: [email protected]
- Phone: +55 (11) 2021-2372.

**Warranty:**
Factory warranty against manufacturing defects, not covering misuse or improper installation.

Images are for illustrative purposes only and may not represent reality.